Abstract
Stroke is a devastating and potentially fatal complication to sickle cell Anemia. Strokes are difficult to explain on the basis of the central pathological process in SCA, namely the occlusion of small vessels by deformed sickled cells. We examined whether Single Nucleotide Polymorphism (SNP) variants in the MCP-1 or CCR2 genes independently or in combination are associated with occurrence of Cerebrovascular Accidents (AVC) in SCA Tunisian patients. Material and methods: 100 SCA patients among whom 19 have AVC were enrolled in this study. Clinical diagnosis of stroke was performed by the use of Transcranial Doppler ultrasonography (TCD). The genotyping of rs1026611 in the MCP-1 gene and V64I of CCR2 was performed using PCR/RFLP. Results: Our findings showed no association of the polymorphisms studied with occurrence of AVC in SCA Tunisian patients.
Highlights
Stroke remains one of the important complications of SCA and is especially critical in the care of children with this disorder
Our findings showed no association of the polymorphisms studied with occurrence of AVC in SCA Tunisian patients
Monocyte chemo attractant protein 1 (MCP-1) is a member of the C–C beta chemokine family that is produced by macrophages, fibroblasts, and endothelial cells to stimulate chemo taxis of monocyte/ macrophages and other inflammatory cells
Summary
Stroke remains one of the important complications of SCA and is especially critical in the care of children with this disorder. The hypothesis of modifier gene in SCA can help the researchers to understand this disease [2]. We focused on two chemokine’s namely Monocyte chemo attractant protein 1 (MCP-1), with its receptor chemokine receptor 2 (CCR2). MCP-1 is a member of the C–C beta chemokine family that is produced by macrophages, fibroblasts, and endothelial cells to stimulate chemo taxis of monocyte/ macrophages and other inflammatory cells. The human MCP-1 regulates the infiltration of monocytes, memory T cells and macrophages and other inflammatory cells by binding to the membrane CC chemokine receptor 2 (CCR2) [5,6,7,8]. MCP-1 protein may be regulated by a Single Nucleotide Polymorphism (SNP) occurring at position −2518 of the MCP-1 gene promoter. Numerous studies have been performed on the association of the -2518 A/G Polymorphisms in the MCP-1 gene with atherosclerosis susceptibility
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
