Abstract
BackgroundPromyelocytic leukaemia zinc finger (PLZF) is a transcriptional repressor that was originally isolated from a patient with promyelocytic leukaemia. PLZF also affects key elements for cell cycle progression, such as cyclin A, and can affect the tumourigenicity of various cancers. Thus far, the behaviour of PLZF in thyroid carcinoma remains unclear.MethodsWe analysed the expression profile of PLZF in different types of benign and malignant thyroid lesions as well as in normal thyroid tissue. Specifically, we examined PLZF expression in normal thyroid (N; n = 4), adenomatous lesion (AL; n = 5), follicular adenoma (FA; n = 2), papillary thyroid carcinoma (PTC; n = 20), and anaplastic thyroid carcinoma (ATC; n = 3) samples. PLZF expression was estimated by western blotting and immunohistochemical (IHC) staining.ResultsPLZF was expressed in all samples of thyroid lesions examined. In N, AL, and FA, PLZF was mainly localized in the nucleus. In contrast, in PTC and ATC, PLZF was mainly expressed in the cytosol with high intensity. In more detail, the cytoplasmic IHC scores in PTC with capsular invasion (CI) and lymph node (LN) metastasis were higher than those in PTC without CI and LN metastasis.ConclusionsPLZF shows different subcellular localizations among PTC, ATC, and other thyroid lesions. Furthermore, high cytoplasmic expression of PLZF may be correlated with CI and LN metastasis in thyroid carcinoma. The present report is the first to describe the implications of intracellular PLZF expression in thyroid carcinomas.
Highlights
Promyelocytic leukaemia zinc finger (PLZF) is a transcriptional repressor that was originally isolated from a patient with promyelocytic leukaemia
Promyelocytic leukaemia zinc finger (PLZF), which was first identified as a partner gene fused to retinoic acid receptor alpha in the variant chromosomal translocation t(11;17)(q23;q21) in acute promyelocytic leukaemia, is a nuclear transcription factor belonging to the BTB/POZ family [4,5,6]
The PLZF expression levels were relatively low in N and adenomatous lesion (AL) compared with papillary thyroid carcinoma (PTC) (Figure 1A)
Summary
Promyelocytic leukaemia zinc finger (PLZF) is a transcriptional repressor that was originally isolated from a patient with promyelocytic leukaemia. The behaviour of PLZF in thyroid carcinoma remains unclear. There is a compelling need for better understanding of Promyelocytic leukaemia zinc finger (PLZF), which was first identified as a partner gene fused to retinoic acid receptor alpha in the variant chromosomal translocation t(11;17)(q23;q21) in acute promyelocytic leukaemia, is a nuclear transcription factor belonging to the BTB/POZ family [4,5,6]. Wild-type PLZF has been directly linked to tumour suppression via its transcriptional repression of the c-myc oncogene [9].
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