Implication of gamma delta T cells in the immune response against murine CMV

Abstract

Event Abstract Back to Event Implication of gamma delta T cells in the immune response against murine CMV Camille Khairallah1, Sonia Netzer1, Arnaud Villacreces1, Marina Juzan1, Vincent Praloran1, Jean-François Moreau1, Julie Déchanet-Merville1 and Myriam Capone1* 1 CNRS, Immunology, France We previously showed that gamma delta T cells are key contributors to the immune response against CMV in humans, and participate to CMV riddance partially through interferon gamma (IFNg) release. The aim of the present study was to use the mouse model of CMV infection in order to dissect in vivo the implication of murine gamma delta T cells in the control of mouse CMV (MCMV). Using C57BL/6 mice deficient for alpha beta and/or gamma delta T cells, we showed that whichever subpopulation is sufficient to confer protection against MCMV. Viral loads increased with time in all organs tested from CD3epsilon-/- (alpha beta- gamma delta-) infected mice, and the presence of high levels of transaminases in blood was suggestive of liver damage. CD3epsilon-/- ultimately died unlike TCRalpha-/- (alpha beta- gamma delta+) mice that survived with much lower viral loads. In TCRalpha-/- mice, CD27+ gamma delta T cells were increased in the liver 7 days post MCMV-infection suggesting an implication of IFNg-secreting gamma delta T cells in the clearance of MCMV in this organ. Finally, bone marrow transfer experiments using TCRalpha-/- donors rendered CD3epsilon-/- mice resistant to MCMV in accordance with a protective anti-viral role for gamma delta T cells. These results which suggest that gamma delta T cells could compensate for the absence of alpha beta T cells during MCMV infection could be of particular relevance in immune-suppressive contexts where alpha beta T cells are more specifically compromised. Keywords: Gamma Delta T cells, CMV infection, CD27, IFN- γ, mouse model Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Khairallah C, Netzer S, Villacreces A, Juzan M, Praloran V, Moreau J, Déchanet-Merville J and Capone M (2013). Implication of gamma delta T cells in the immune response against murine CMV. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00948 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Myriam Capone, CNRS, Immunology, Bordeaux, 33076, France, mcapone@cirid.org Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Camille Khairallah Sonia Netzer Arnaud Villacreces Marina Juzan Vincent Praloran Jean-François Moreau Julie Déchanet-Merville Myriam Capone Google Camille Khairallah Sonia Netzer Arnaud Villacreces Marina Juzan Vincent Praloran Jean-François Moreau Julie Déchanet-Merville Myriam Capone Google Scholar Camille Khairallah Sonia Netzer Arnaud Villacreces Marina Juzan Vincent Praloran Jean-François Moreau Julie Déchanet-Merville Myriam Capone PubMed Camille Khairallah Sonia Netzer Arnaud Villacreces Marina Juzan Vincent Praloran Jean-François Moreau Julie Déchanet-Merville Myriam Capone Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.