Implication of exogenous abscisic acid (ABA) application on phytoremediation: plants grown in co-contaminated soil.

Abstract

Abscisic acid (ABA) may play an important role in alleviating negative effects of heavy metal stress on growth performance of plants. A pot experiment was conducted to investigate differential effects of exogenous ABA with different concentrations (0, 20, 40, and 60 μmol/L) on heavy metal accumulation and physiological response of Cd/Zn hyperaccumulator Sedum alfredii Hance and non-hyperaccumulator Hylotelephium spectabile (Boreau) H. Ohba grown in co-contaminated soil. In the experiment, Cd, Zn, or Pb concentration in stem and leaf of H. spectabile was significantly increased by exogenous ABA application than control. However, the opposite pattern was observed for S. alfredii. With decrease of Cd concentration, Zn or Pb concentration in root of H. spectabile grown in co-contaminated soil was significantly increased by exogenous ABA application than control. Cd, Zn, or Pb concentration in root of S. alfredii was significantly increased by exogenous ABA application than control. Compared with S. alfredii, BCF and TF of Cd, Zn, or Pb for H. spectabile were significantly increased by exogenous ABA application. With negative effect on root growth, total biomass of the two species, especially H. spectabile, was significantly increased by exogenous ABA application than control. With increase of their total chlorophyll content, antioxidant capacity of the two species subjected to heavy metal stress was improved by exogenous ABA application than control. Heavy metal-induced growth inhibition was significantly alleviated by exogenous ABA application when the two species were grown in co-contaminated soil. We tentatively concluded that differential effects of exogenous ABA application on transport pathway of ions incurred different patterns of heavy metal accumulation between Cd/Zn hyperaccumulator S. alfredii and non-hyperaccumulator H. spectabile. It is suggested that compared with Cd/Zn hyperaccumulator S. alfredii, exogenous ABA application may improve heavy metal uptake in root and transport of heavy metal ions between different organs for non-hyperaccumulator H. spectabile grown in co-contaminated soil. Our results provide insight into effects of exogenous ABA application on phytoremediation of Cd-, Pb-, and Zn-co-contaminated soil.