Abstract
A glucanase [(1→3)-β- d-glucan glucanohydrolase EC 3.2.1.6, laminarinase] detected in healthy melon seedlings ( Cucumis melo L.) increased greatly in activity 48 h after infection by Colletotrichum lagenarium (Pass.) Ell. and Halst. When grown in a heterogeneous medium containing 0·5% isolated cell walls from melon fruits as nitrogen and carbon sources this fungus secreted β-(1→3) glucanases (exo- and endo-forms). By means of molecular filtration, ion-exchange chromatography, thermostability and studies of enzymatic reaction products we showed that the increase of laminarinase activity in infected tissues was caused by an endoenzyme form produced by C. lagenarium. In this disease, host β-(1→3) glucanase does not provide a defense mechanism against C. lagenarium.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
