Abstract
Slaughter products are good source of bioactive substances with different biological effects. Nowadays, approximately 220 functional peptides consisting of 2–10 amino acid residues have been identified. In this study, the biological activity of medium-molecular-weight substances (5–30 kDa, Mw) and low-molecular-weight substances (less than 5 kDa, Mw) extracted from aorta of Sus scrofa were investigated in vitro. Medium-molecular-weight and low-molecular-weight fractions possessed a tissue specific effect on organotypic tissue cultures of chicken embryos and laboratory rats. Biological activity depended on molecular weight. Medium-molecular-weight fractions possessed higher effect on aortas explants of chicken embryo: area index of cellular monolayer was 56.6±5.2 % (area index of the monolayer was 37.47±3.27 % for low-molecularweight fraction) in comparison with control explants. The low-molecular-weight fractions stimulated aortic tissues explants of aging rats — area index exceeded area index formedium-molecularweight fractions by more than 30%.
Highlights
Slaughter products are good source of bioactive substances with different biological effects
Explants of the chicken embryo and rat tissues wereanalyzed after 24 hours of cultivation in a culture medium with addition of the test samples
It was noticed that area indices of cardiac and aortic tissues explants of chicken embryos with addition of the test samples were different
Summary
Slaughter products are good source of bioactive substances with different biological effects. Medium-molecular-weight and low-molecular-weight fractions possessed a tissue specific effect on organotypic tissue cultures of chicken embryos and laboratory rats. Medium-molecular-weight fractions possessed higher effect on aortas explants of chicken embryo: area index of cellular monolayer was 56.6±5.2 % (area index of the monolayer was 37.47±3.27 % for low-molecularweight fraction) in comparison with control explants. Что куриный миозин, говяжий коллаген α1, свиной тропонин С обладают широким спектром активностей. Показано, что гипотензивные последовательности высвобождаются при гидролизе миозиновой тяжелой цепи куриного бедра и коллагена, говяжьих саркоплазматических белков, свиного небулина, миозина (тяжелая цепь), актина, тропонина С и титина, говяжьего, свиного и куриного тропонина С сердечной мышцы и пр. Что последовательности, обладающие антиоксидантными свойствами, высвобождаются при гидролизе говяжьих коллагена α1, тропомиозина α1, саркоплазматических белков печени, свиных миофибриллярных белков, куриного белка и говяжьей крови. В частности, выявлено, что сыровяленые свиные колбасы, в частности, испанская «Чоризо», кантонская колбаса и хорватской «Петровац», в водорастворимой фракции белков содержат множество антиоксидантных пептидов [5]
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