Implementation of flow cytometry in the diagnostic work-up of myelodysplastic syndromes in a multicenter approach: Report from the Dutch Working Party on Flow Cytometry in MDS

T.m Westers ,Roelof Bekkema,Claudia Cali,Arjan A Van De Loosdrecht,Jeroen G Te Marvelde,Canan Alhan,Bijan Moshaver,Alita Van Der Sluijs,Rik A Brooimans,Christa Homburg,Vincent H.J Van Der Velden,Roger K Schindhelm,André B Mulder ,A Bijkerk ,I Lommerse ,P.a Kuiper-Kramer ,Frank Preijers ,J Van Der Molen-Sinke ,A.m Dräger ,Anneke De Jong ,Valérie De Haas ,A.h Westra ,E.r Van Wering ,Marij Leenders

doi:10.1016/j.leukres.2011.09.015

Implementation of flow cytometry in the diagnostic work-up of myelodysplastic syndromes in a multicenter approach: Report from the Dutch Working Party on Flow Cytometry in MDS

T.m Westers , Roelof Bekkema + Show 22 more

https://doi.org/10.1016/j.leukres.2011.09.015

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Journal: Leukemia Research	Publication Date: Oct 6, 2011
Citations: 35

Affiliation: University Medical Center Groningen, Amsterdam UMC Location VUmc, Erasmus University Rotterdam, Erasmus MC, Isala, Radboud University Nijmegen, Stichting Kinderoncologie Nederland, Sanquin

#Myelodysplastic Syndromes #Diagnostic Work-up Of Myelodysplastic Syndromes + Show 8 more

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Abstract

Flow cytometry (FC) is recognized as an important tool in the diagnosis of myelodysplastic syndromes (MDS) especially when standard criteria fail. A working group within the Dutch Society of Cytometry aimed to implement FC in the diagnostic work-up of MDS. Hereto, guidelines for data acquisition, analysis and interpretation were formulated. Based on discussions on analyses of list mode data files and fresh MDS bone marrow samples and recent literature, the guidelines were modified. Over the years (2005–2011), the concordance between the participating centers increased indicating that the proposed guidelines contributed to a more objective, standardized FC analysis, thereby ratifying the implementation of FC in MDS.

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