Abstract
Sulfation made easy: Syntheses of tyrosine-sulfated proteins were developed by coupling an in situ 3′-phosphoadenosine-5′-phosphosulfate (PAPS)-generating system and tyrosylprotein sulfotransferase (TPST) catalysis. High catalytic efficiency of TPST in vitro was demonstrated, and by using a similar strategy, sulfated proteins were produced in vivo through bacterial cultivation.
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