Impedimetric Lectin Biosensor for Prostate Cancer Detection

Abstract

There is considerable attention on the determination of prostate-specific antigen (PSA) glycosylation patterns for the early detection of prostate cancer, as it is one of the most reliable tumor biomarkers. In this study, the highly effective lectin-based biosensor utilizing gold interdigitated microelectrode was developed as a sensing transducer, coupled with electrochemical impedance spectroscopy (EIS) for the sensitive detection of biomolecules event on the device. The self-assembled monolayer using 11-mercaptoundecanoic acid was utilized to modify the sensor surface for the conjugation of Maackia amurensis lectin as biorecognition elements. The analytical analysis of the developed lectin biosensor with PSA glycosylation through impedimetric measurement exhibited a linear detection ranging from 100 pg/mL to 100 ng/mL and attained a detection limit of 27.6 pg/mL.