Abstract
An aptasensor is described for the ultrasensitive detection of Pseudomonas aeruginosa (P. aeruginosa). A glassy carbon electrode (GCE) was first modified by electrodeposition of silver nanoparticles (AgNPs). Immobilization of NH2-aptamer was covalently attached to the AgNP/GCE surface. The morphology, distribution and size of the sensor were characterized by field emission scanning electron microscopy. Cyclic voltammetry and electrical impedance spectroscopy were used to study conductivity of the aptasensor and the electrochemical properties. Detection of P. aeruginosa was carried out by evaluation of the charge transfer resistance after and before the adding of P.aeruginosa and by using the hexacyanoferrate redox system as an electrochemical probe. The impedance increases on going from 102 to 107CFU·mL-1 concentrations of P. aeruginosa, and the detection limit is 33CFU·mL-1 (for S/N= 3). The assay was successfully applied for the determination of P. aeruginosa in spiker serum samples. Graphical abstract Schematic representation of animpedimetric assay for Pseudomonas aeruginosa by using a [Fe(CN)6]3-/4- probe based on immobilization of amino-modifiedaptamer onto a glassy carbon electrode (GCE) modified with silver nanoparticles (AgNPs): Incubation with P.aeruginosa leads to anincrease of the charge-transfer resistance.
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