Abstract
Thermal analysis of suspension impedance was applied at various frequencies, f, to study the changes in erythrocyte membranes (EM) over the temperature interval 47–53 °C where spectrin denatures. At f < crossover frequency (cof1) a decrease in EM resistance at 49.5 °C (A peak) has been observed. At f > cof1, when the capacitive conductance of EM becomes dominant, a second peak appeared at 50.5 °C (anti-A peak). The amplitudes of A and anti-A peaks reversibly depended on the phosphorylation of EM proteins. In contrast to the A peak, the anti-A peak was totally eliminated by modifications of EM with reagents that reduce EM deformability, O sO 4 (0.4–1 mM) and putrescine (cytosolic concentration 1.5–2.0 mM).
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