Abstract

ABSTRACTAn impedance procedure was developed for rapid screening of low acid foods. Impedance Medium was used during the pre‐enrichment step and the capacitance monitoring stage using the Bactometer®. Thermophiles and mesophiles were detected by incubating at 55°C and 35°C. Anaerobes were detected by either placing the Bactometer® in an anaerobic chamber or adding an oxygen reducing membrane fraction directly to the medium. Naturally contaminated foods containing approximately 104 CFU/g resulted in detections within 8 hr. Artificially contaminated products with 104 CFU/g resulted in detections within 24 hr (6 hr pre‐enrichment inclusive). This procedure screened low‐acid foods within 24 hr, faster than the conventional methodology.

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