Abstract
Si/SiO 2 chips were used to detect the hybridization of immobilized Oligo d(T) 20 through impedance measurement. The immobilization procedure involved an aminopropyl silane grafted silicon oxide surface activated by glutaraldehyde and subsequently modified by an aminolinker supporting oligonucleotides. The immobilization procedure was optimized and, in the best conditions, the hybridization of the immobilized oligonucleotide was able to generate a 50 Ω impedance change at an applied dc potential of −300 mV. The optimized DNA sensor was then used to directly determine the immobilized oligonucleotide T m via impedance measurement in a continuous temperature control flow system. A reproducible and specific 65 Ω impedance change was observed at 32 °C with a step duration as low as 15 min. This value compared well with the 31.4 °C theoretical value calculated from the sequence base pair composition and length.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
