Abstract
BackgroundCD90 (Thy-1) is a small glycoprotein that is particularly abundant on the surface of mouse thymocytes and peripheral T cells, and is often used as a marker in adoptive transfer experiments to distinguish donor and recipient T cells with different CD90 subtypes. We have performed adoptive transfer experiments with T cell receptor transgenic (TCR Tg) mice to study the impaired CD8 T cell response with aging.FindingsAfter stimulation with a CD8 T cell epitope, HA518-524, the response of TCR Tg CD8 T cells from aged mice was decreased compared to the response of TCR Tg T cells from young mice. CD90 expression was also substantially decreased on the TCR Tg CD8 T cells of aged mice. However, the responses of CD90hi and CD90low CD8 T cells of the aged mice were similar in both early activation and proliferation, demonstrating that the impaired Tg T cell response with aging is not associated with the altered CD90 expression on CD8 T cells.ConclusionsThe impaired Tg CD8 T cell response in aged mice is not due to age-associated changes in CD90 expression on Tg CD8 T cells.
Highlights
CD90 (Thy-1) is a small glycoprotein that is abundant on the surface of mouse thymocytes and peripheral T cells, and is often used as a marker in adoptive transfer experiments to distinguish donor and recipient T cells with different CD90 subtypes
The development of new immunological techniques, including T cell receptor transgenic (TCR Tg) mice [6,7,8], specific MHC tetramer staining [9], and lymphocyte sorting with flow cytometry, have significantly accelerated research concerning T cell immunity with aging [2,3,10]
Using an adoptive transfer approach, we observed that the aged environment significantly inhibits both clonal expansion and IFN-γ production by specific Tg CD8 T cells of young mice during virus infection [3], and that the decreased response of the Tg CD8 T cells transferred into aged mice could be significantly enhanced when DCs of young mice were co-transferred [11]
Summary
CD90 (Thy-1) is a small glycoprotein that is abundant on the surface of mouse thymocytes and peripheral T cells, and is often used as a marker in adoptive transfer experiments to distinguish donor and recipient T cells with different CD90 subtypes. Conclusions: The impaired Tg CD8 T cell response in aged mice is not due to age-associated changes in CD90 expression on Tg CD8 T cells. Indicate that alterations in the aged environment play an important role in the decreased specific CD8 T cell immunity to virus infection with aging.
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