Impacts of sublethal concentrations of 17 α-ethinylestradiol (EE2) on growth, reproductive performance, and survival in red cherry shrimp Neocaridina davidi (Crustacea, Atyidae) during consecutive spawnings

Abstract

This study was conducted for the first time to investigate the long-term impacts of sublethal concentrations of 17 α-ethinylestradiol (EE2) on growth, survival, and reproductive performances in a model shrimp, the red cherry (Neocaridina davidi), females during five successive spawning steps for 7.5 months. Females were distributed in eighteen aquariums and continuously exposed to EE2 at six nominal concentrations of 0 (control), 0.02, 0.2, 2, 20, and 200 μg/L. Growth indices increased up to 0.2 μg/L and then sharply declined up to 200 μg/L. Most reproductive indices significantly decreased at levels > 0.02–0.2 μg/L with increasing EE2 levels. The highest absolute, relative, and actual fecundity values were recorded in the control, with the lowest value at 200 μg/L. With increasing EE2 levels, mean egg volume showed an increasing trend from the third spawning event onwards. Except for the time required to reach the first spawning, inter-spawning intervals considerably decreased with increasing EE2 levels at > 0.2 μg/L, especially from the third spawning stage onwards. Survival of exposed females significantly decreased with increasing EE2 levels. Unlike the body size, the juvenile's survival rates in all exposed treatments were considerably lower than the control. Females at concentrations 0.02–0.2 μg/L gained more body weight and length but produced fewer eggs with lower hatching percentages during five consecutive spawns. The results suggest that EE2 depending on the concentrations can cause unbalanced growth, reduce reproductive performances, especially from the third stage of spawning onwards, and reduce survival rates in brooders and subsequent offspring. In terms of growth, survival, and reproductive indices over successive spawns in ecotoxicology studies, the concentrations of 0.02–0.2 μg/L can be considered as chronic levels, but higher levels may have detrimental effects.