Impacts of acute toxicity of arsenic (III) alone and with high temperature on stress biomarkers, immunological status and cellular metabolism in fish

Abstract

The water bodies are greatly influenced by heavy metal contamination and global increasing temperature. Arsenic (As) is one of the most dangerous widespread pollutants that pose health threats to human, animals and fishes. Considering the above, the study has been carried out to delineate 96 h median lethal concentration of arsenic alone and in combination with high temperature (As-T, 34 °C) by conducting static non-renewable bio-assay acute toxicity in Pangasianodon hypophthalmus (average weight 6.25 ± 0.69 g, length 5.32 cm). Effect of definitive doses such as 25, 26, 27, 28, 29 and 30 mg/L of As alone and in combination with high temperature (As-T) were evaluated on stress biomarkers and cellular metabolism of P. hypophthalmus. The lethal concentration (96 h LC50) of As alone and in combination with high temperature was found to be 28.16 mg/L and 26.88 mg/L, respectively. The stress biomarkers in terms of catalase, superoxide dismutase (SOD) and glutathione-s-transferase (GST) in liver, gill, brain and kidney, blood glucose and NBT were remarkable higher (p < 0.01) in comparison to unexposed group (control group). Brain neurotransmitter enzyme, AChE, immunological status (blood glucose and NBT) and cellular metabolic enzymes (lactate dehydrogenase LDH, malate dehydrogenase MDH, aspartate aminotransferase AST, and alanine aminotransferase ALT, glucose-6-phosphate dehydrogenase G6PDH and ATPase) were noticeably (p < 0.01) altered by As and As-T exposure. The histopathological study exhibited devastating changes with exposure to As and As-T such as bile stagnation, hepatocyte with irregular nucleus, eosinophilic granules in the cytoplasm, necrosis, and nuclear hypertrophy in liver and curling of secondary lamellae, hypertrophy of lamellar epithelium, blood congestion, incomplete fusion of secondary lamellae, complete fusion of several lamellae and aneurysm in gill. Overall results clearly indicate that acute exposure of As and high temperature led to pronounced deleterious alterations on stress biomarkers and cellular and metabolic activities of P. hypophthalmus.