Impact of water temperature on the shipping of berried female broodstock (Macrobrachium rosenbergii): A study on survival, egg retention, hatching duration, and hatching success rate

Abstract

This study evaluated the impact of different water temperatures on shipping berried female Macrobrachium rosenbergii broodstocks. The broodstocks, averaging 23±0.07 g in weight and 12±0.1 cm in length, were shipped in styrofoam boxes containing 24L of purified freshwater. Three temperature conditions were tested: 28.5±0.5oC (control), 25.5±0.5oC (T1), and 21.5±0.5oC (T2). Various water quality parameters were measured. Mortality rates were significantly lower in T2 (2.60±0.41%) and T1 (8.96±2.09%) compared to the control (24.89±4.96%) (p<0.05). The percentage of healthy berried females was higher in T2 (64.18±3.77%) and T1 (56.12±0.59%) than in the control (39.43±2.44%) (p<0.05). T2 exhibited the lowest egg weight loss (6.17±0.66 g) and the shortest hatching period (26.33±3.30 hrs) with the highest hatching success rate (90.68±0.34%) (p<0.05). Dissolved oxygen levels decreased more in the control group compared to T1 and T2 (p<0.05). Total nitrite levels increased significantly in the control group compared to T1 and T2 (p<0.05). The study concludes that cooler shipping temperatures, particularly around 21.5oC, benefit the survival, health, and hatching performance of berried female M. rosenbergii broodstocks.