Abstract
The work provides experimental data on the impact of anticoagulants (K3 - EDTA, 3.8% sodium citrate and lithium heparin) and coagulation activator on morpho-biochemical indicators in the blood samples of broiler chickens of cross-selection of Agricultural Center “Smena” at the age of 35 days. The studies were carried out with the use of semi-automatic flow analyzer Sinnowa BS-3000P (SINNOWA Medical Science & Technology Co., Ltd, China) and veterinary automatic blood analyzer DF-50 by Dymind Biotech (PRC) with the use of original reagents. The results showed that the most optimal reagent for determination of morphological indicators of the poultry blood was EDTA. The average white blood count (WBC) is lower in blood samples with citrate and heparin in contrast to EDTA by 30.5% and 24.1%, respectively. The average red blood count (RBC) is lower in tubes with sodium citrate (by 37.9 %) and lithium heparin (by 13.8 %) in contrast to K3-EDTA. When the sodium citrate anticoagulant is used, the blood biochemistry results for all positions of the experiment, excluding calcium, are lower than in heparin, and its closest values are lower than in coagulation activator, excluding trypsin activity. That’s why, the most optimal reagents for biochemistry, including for blood enzyme tests, should be considered the tubes with heparin or coagulation activator.
Highlights
The morphological and biochemical blood indicators are important for determining the physical status and health of the live-stock animals and poultry, including for mycotoxicosis diagnostics
The purpose of this work was to compare the impact of anticoagulants (K3 - EDTA, 3.8% sodium citrate and lithium heparin) on morpho-biochemical indicators in the blood samples of broiler chickens of cross-selection of Agricultural Center “Smena” at the age of 35 days
The tests were performed with simultaneous use of different tubes: with K3 EDTA, lithium heparin, sodium citrate and coagulation activator
Summary
The morphological and biochemical blood indicators are important for determining the physical status and health of the live-stock animals and poultry, including for mycotoxicosis diagnostics. The existing anticoagulants ensure the preservation of the components without significant changes in the course of sample analysis and are characterized by the properties that inhibit the blood components coagulation process This prevents blood coagulation due to the binding of calcium ions (EDTA, sodium citrate), inhibition of thrombin activity (heparin, hirudin) [2,3]. There is enough information in the literature on the effect of various types of anticoagulants on hematological indicators in human or animals, but there is limited information on the use of tubes for studying blood samples of poultry In this regard, in order to obtain more correct analysis results, it is important to choose the correct anticoagulant for blood collection. The purpose of this work was to compare the impact of anticoagulants (K3 - EDTA, 3.8% sodium citrate and lithium heparin) on morpho-biochemical indicators in the blood samples of broiler chickens of cross-selection of Agricultural Center “Smena” at the age of 35 days
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