Impact of two commercially available hair dyes on germination, morpho-physiology, and biochemistry of Cicer arietinum L. and cytotoxicity study on Allium cepa L. root tip

Abstract

Contamination of agricultural land and surface water by personal care products and pharmaceutical constituents is a potential environmental threat. The active ingredients of personal care products are life-threatening for users. Present work highlighted the efficacy of the different components of two commercially available hair dyes (synthetic and herbal) on germination, morpho-physiological, biochemical parameters of Cicer arietinum, and cytotoxicity study by Allium cepa root tip. Different treatments such as T1 (control), T2 (cream colour rich), T3 (developer) (The ingredients T2 and T3 are from the same hair dye), T4 (an equal mixture of T2 and T3), and T5 (herbal dye) were considered to run this experiment. The results revealed that all the treatments improve germination with respect to control. Moreover, GSI data suggests that T2 showed the highest germination speed and T3 showed the lowest with respect to other treatments. But root lengths are severely affected by the treatments T3 (100% developer of synthetic hair dye), T4 (an equal mixture of T2 (100% cream colour rich) and T3), and T5 (100% herbal hair dye) with respect to control.T2 also showed the highest root tolerance of all treatments other than control. Similarly, one-way ANOVA results revealed that both fresh weight of roots (p ≤ 0.03) and shoots (p ≤ 0.03) are statistically significant among the different treatments. Moreover, both proline and root ion leakage are higher in the treatment T4 and T5 with respect to control, respectively. On the other hand, the cytotoxicity study highlighted that treatments T3 and T4 showed a higher level of aberration and significantly lower mitotic index compared to treatment T5. Therefore, finally, it may be concluded that both individual and combined forms of ingredients of hair dyes are toxic with respect to cell division and overall plant growth and development.