Impact of transparent tray-based application of bioactive glasses desensitizer on the permeability of enamel and dentin to hydrogen peroxide: an in vitro study

Qian Ma,Xiao Xu,Tianda Wang,Jingwen Chen

doi:10.1186/s12903-020-01096-5

Abstract

BackgroundTo investigate the effect of transparent tray-based application of bioactive glasses (BGs) desensitizer on the permeability of enamel and dentin to hydrogen peroxide (H2O2).MethodsFreshly extracted human first premolars were divided into 6 groups (n = 8). Group A and B: without pretreatments; Group C and E: treated with BGs desensitizer only; Group D and F: treated with BGs desensitizer dispensed with a transparent tray. After roots and pulp tissues of the treated tooth specimens were thoroughly removed, acetate buffer was added into pulp chambers and the treated specimens were immersed in distilled water (Groups A, E, and F) or 30% H2O2 (Groups B, C, and D) for 30 min at 37 °C. The amount of H2O2 in the pulp chamber of each group was measured using ultraviolet-visible spectrophotometry.ResultsIn control groups (Group A, E, and F), H2O2 was not detected. The amount of pulpal H2O2 in Group B, C, and D were 21.149 ± 0.489 μg, 9.813 ± 0.426 μg, and 4.065 ± 0.268 μg respectively. One-way ANOVA analysis indicated that significant differences existed in these groups (F = 459.748, p < 0.05).ConclusionsThe effect of BGs desensitizer in reducing the permeability of enamel and dentin to H2O2 could be enhanced when dispensed with a transparent tray.

Highlights

To investigate the effect of transparent tray-based application of bioactive glasses (BGs) desensitizer on the permeability of enamel and dentin to hydrogen peroxide (H2O2)
Peroxides such as hydrogen peroxide and carbamide peroxide used to bleach discolored dental hard tissues have been shown to have the ability to penetrate enamel and dentin, which is determined by several factors including the concentration of peroxides, the exposure time of teeth to peroxides, cracks and restorative
Surface treated with BGs desensitizer only, immersed in 30% Hydrogen peroxide (H2O2)

Summary

Introduction

To investigate the effect of transparent tray-based application of bioactive glasses (BGs) desensitizer on the permeability of enamel and dentin to hydrogen peroxide (H2O2). After roots and pulp tissues of the treated tooth specimens were thoroughly removed, acetate buffer was added into pulp chambers and the treated specimens were immersed in distilled water (Groups A, E, and F) or 30% H2O2 (Groups B, C, and D) for 30 min at 37 °C. Whitening could be achieved either by physically removing discolored dental hard tissues or chemically bleaching stains. Compared with resin or ceramic veneers and all-ceramic crowns which could physically replace discolored hard tissues, chemically tooth bleaching is considered to be the simplest, most cost-effective and. Peroxides are highly reactive and could convert to superoxide (O2−), hydroxyl (HO-), peroxyl (ROO-) or alkoxyl (RO-) radicals in physical environments. These oxidative oxygen species can freely penetrate dental

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Conclusion