Abstract
BackgroundThe zootechnical performance of three different commercial feeds and their impact on liver and serum proteins of gilthead sea bream (Sparus aurata, L.) were assessed in a 12 week feeding trial. The three feeds, named A, B, and C, were subjected to lipid and protein characterization by gas chromatography (GC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively.ResultsFeed B was higher in fish-derived lipids and proteins, while feeds C and A were higher in vegetable components, although the largest proportion of feed C proteins was represented by pig hemoglobin. According to biometric measurements, the feeds had significantly different impacts on fish growth, producing a higher average weight gain and a lower liver somatic index in feed B over feeds A and C, respectively. 2D DIGE/MS analysis of liver tissue and Ingenuity pathways analysis (IPA) highlighted differential changes in proteins involved in key metabolic pathways of liver, spanning carbohydrate, lipid, protein, and oxidative metabolism. In addition, serum proteomics revealed interesting changes in apolipoproteins, transferrin, warm temperature acclimation-related 65 kDa protein (Wap65), fibrinogen, F-type lectin, and alpha-1-antitrypsin.ConclusionsThis study highlights the contribution of proteomics for understanding and improving the metabolic compatibility of feeds for marine aquaculture, and opens new perspectives for its monitoring with serological tests.Electronic supplementary materialThe online version of this article (doi:10.1186/s12953-014-0044-3) contains supplementary material, which is available to authorized users.
Highlights
The zootechnical performance of three different commercial feeds and their impact on liver and serum proteins of gilthead sea bream (Sparus aurata, L.) were assessed in a 12 week feeding trial
It is worth noting that Feed A showed high contents of both docosahexaenoic acid (DHA) and eicosapentanoic acid (EPA), which is in keeping with a substantial supplementation of fish oil to the vegetable matrix
This study presents a comprehensive evaluation of the impact of three commercial feeds, designated as A, B, and C, on gilthead sea bream growth and metabolism, carried out by assessing protein abundance changes in liver tissue and blood serum at the end of a 12 week feeding trial (T0 vs T12A, T12B, and T12C, respectively)
Summary
The zootechnical performance of three different commercial feeds and their impact on liver and serum proteins of gilthead sea bream (Sparus aurata, L.) were assessed in a 12 week feeding trial. The high costs and the unsustainable overexploitation of the wild fish stock have generated an increasing demand for developing feed formulations incorporating alternative raw materials, involving the replacement of fish turn, will attract consumer interest in higher level products To this aim, sustainable and cheap protein sources, such as meat byproducts including blood meal, bone meal and, more recently, poultry or porcine waste matter, can integrate plant-based meals with some of the essential nutrients present only in fish meal and not in vegetable sources. Insights into the response of fish metabolism to dietary substitutions can be provided by investigating how the proteome of pivotal tissues of the organism is affected by nutritional changes [12,13] With this purpose, numerous research groups have applied proteomic analysis to farmed fish biofluids or tissues such as serum, liver, muscle, and other organs [12,13,14,15,16,17,18,19,20,21]. Other authors investigated the liver proteome changes in gilthead sea breams exposed to low temperatures [22]; liver proteomics was used to study the influence of handling and crowding as chronic stressors [18] and to study the effects of different contaminants, antibacterial, and antiparasitic agents [23,24]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
