Abstract
To date, metal-organic frameworks (MOFs) have been recognized as promising solid phases in high-performance liquid chromatography (HPLC). This research aimed to elucidate the role of the physico-chemical characteristics of the microporous HKUST-1 metal-organic framework in its operation as a selective adsorbent in HPLC. For this, the HKUST-1 samples were prepared by microwave-assisted synthesis and a solvothermal procedure. According to the chromatographic examinations, the HKUST-1 material synthesized in the microwave fields shows an efficient performance in the selective adsorption of aromatic compounds with different functionalities. This study revealed a significant impact of the preparation procedure on the mechanism of the liquid-phase adsorption on the HKUST adsorbents under conditions of the HPLC. An effect of the elution solvent with the different coordination ability to the Cu2+ sites in the HKUST-1 structure on the adsorption selectivity was observed.
Highlights
High-performance liquid chromatography (HPLC) is a powerful tool for the selective and preparative separation of a large variety of organic compounds [1]
The synthesized HKUST-1 samples were characterized by PXRD, SEM, and N2 low temperature
A comprehensive study of the mechanism of the liquid-phase adsorption shows the possibility of the penetration of benzene molecules into the micropore space in the case of the HKUST-1solv.1 material synthesized by the solvothermal technique, and respectively localization of benzene molecules on the surface of pores of the HKUST-1mw sample
Summary
High-performance liquid chromatography (HPLC) is a powerful tool for the selective and preparative separation of a large variety of organic compounds [1]. The geometry of the column material comprises the following characteristics: surface area, pore volume, pore diameter, particle size and shape. The surface area of HPLC adsorbents is regarded as one of the most important parameters, because the retention volume is generally proportional to the surface area accessible for the molecules of a given analyte [3]. Surface area accessibility is dependent on the analyte molecular size, adsorbent pore diameter, and pore size distribution. The particle size, particle shape, particle size distribution, packing density, and packing uniformity impact on the column efficiency and flow resistance. The surface functionality (along with specific surface area) affects the analyte retention and separation selectivity
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