Abstract
To assess the impact of the number of previous miscarriages in couples with ≥2 miscarriages of unknown etiology. Retrospective observational study. A total of 132 CCS cycles were included in women ≤38 years of age, from January 2010 to December 2012. Day-3 embryo biopsies were performed and embryo transfer was scheduled on day-5. A single blastomere from each embryo underwent Whole Genome Amplification (WGA) with Sureplex DNA Amplification System (BlueGnome Ltd, Cambridge, UK). WGA products and genomic DNA used as control were labeled with Cy3 and Cy5 fluorophores. Both labeled DNAs were co-hybridized overnight in the 24sure platform (BlueGnome Ltd, Cambridge, UK). Slides were scanned and analyzed by BlueFuse Multi software (BlueGnome Ltd, Cambridge, UK). Pearson's correlation (p<0.05) was performed using SPSS Statistics for Windows, Version 17.0 (Chicago: SPSS Inc.). Results according to the number of previous miscarriages are shown in the table. No correlation was observed for the described variables.Tabled 12 miscarriages3 miscarriages4 miscarriages≥ 5 miscarriagesNo. of cycles5053236% Transfers88.077.369.6100% Abnormal embryos56.666.465.250.0% Complex aneuploidies*Embryos with aneuploidies for more than one chromosome.11.116.321.520.8% Partial aneuploidies7.34.85.90% Chaotic pattern13.918.310.420.8Pregnancy rate/transfer54.558.568.766.7Miscarriage rate4.220.8012.5Implantation rate50.742.466.740.0Pregnancy rate/cycle48.045.355.066.7* Embryos with aneuploidies for more than one chromosome. Open table in a new tab High pregnancy and implantation rates were achieved with CCS in couples experiencing repeated miscarriages of unknown etiology. No correlation was found with the number of previous miscarriages and the percentage and type of chromosome abnormalities. Reproductive outcome was not affected by the number of previous miscarriages.
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