Impact of the 7-bp deletion in HvGA20ox2 gene on agronomic important traits in barley (Hordeum vulgare L.)

Serafima Teplyakova,Nina Voytsutskaya,Marina Lebedeva,Olga Kovaleva,Valentina Horeva,Elena Potokina,Nadezhda Ivanova

doi:10.1186/s12870-017-1121-4

Abstract

BackgroundAlike to Reduced height-1 (Rht-1) genes in wheat and the semi dwarfing (sd-1) gene in rice, the sdw1/denso locus involved in the metabolism of the GA, was designated as the ‘Green Revolution’ gene in barley. The recent molecular characterization of the candidate gene HvGA20ox2 for sdw1/denso locus allows to estimate the impact of the functional polymorphism of this gene on the variation of agronomically important traits in barley.ResultsWe investigated the effect of the 7-bp deletion in exon 1 of HvGA20ox2 gene (sdw1.d mutation) on the variation of yield-related and malting quality traits in the population of DHLs derived from cross of medium tall barley Morex and semi-dwarf barley Barke. Segregation of plant height, flowering time, thousand grain weight, grain protein content and grain starch was evaluated in two diverse environments separated from one another by 15° of latitude. The 7-bp deletion in HvGA20ox2 gene reduced plant height by approximately 13 cm and delayed flowering time by 3–5 days in the barley segregating DHLs population independently on environmental cue. On other hand, the sdw1.d mutation did not affect significantly either grain quality traits (protein and starch content) or thousand grain weight.ConclusionsThe beneficial effect of the sdw1.d allele could be associated in barley with lodging resistance and extended period of vegetative growth allowing to accumulate additional biomass that supports higher yield in certain environments. However, no direct effect of the sdw1.d mutation on thousand grain weight or grain quality traits in barley was detected.

Highlights

Alike to Reduced height-1 (Rht-1) genes in wheat and the semi dwarfing gene in rice, the sdw1/denso locus involved in the metabolism of the gibberellic acid (GA), was designated as the ‘Green Revolution’ gene in barley
The beneficial effect of the sdw1.d allele could be associated in barley with lodging resistance and extended period of vegetative growth allowing to accumulate additional biomass that supports higher yield in certain environments
Re-sequencing and mapping of HvGA20ox2 gene in Morex/Barke double haploid lines (DHLs) population To prove that Barke is the sdw1.d mutant we resequenced the exon 1 of HvGA20ox2 gene for the barley variety

Summary

Introduction

Alike to Reduced height-1 (Rht-1) genes in wheat and the semi dwarfing (sd-1) gene in rice, the sdw1/denso locus involved in the metabolism of the GA, was designated as the ‘Green Revolution’ gene in barley. The tremendous success of the “Green Revolution” of the XX century was achieved due to the introduction in agriculture the new cereal varieties distinguished by a shortened thick stem, resistant to lodging. These varieties made it possible to change agricultural technology, The Author(s) BMC Plant Biology 2017, 17(Suppl 1):181. Three other sdw alleles have been obtained artificially with the use of mutagens: 1) sdw1.a mutant (originally named sdw1) were induced by X-rays in the Norwegian six-rowed barley cv. Today cultivars with the sdw1.d mutation from Diamant are often described as «sdw1/ denso». 3) sdw1.e named as ‘Risø no. 9265’ was isolated in a M2 generation from the variety Bomi treated by neutrons [7]

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