Abstract
Objective To study the impact of tetramethylpyrazine on genes expression in colitis mice by means of cDNA microarray.Methods Thirty mice were divided into normal control group,0.9% NaCI solusion group and tetramethylpyrazine group with 10 in each group.Except the mice in normal group,all mice were treated with oxazolone enema,mRNA was extracted from colons of 0.9% NaCI solusion group and tetramethylpyrazine group.The eDNA probes were prepared and labeled with dyes Cy3 or Cy5.Then the fluorescent probe was hybridized with eDNA microarray and scanned for fluorescent intensity.The differently expressed genes were identified by analysis of gene expression profile.Two differently expressed genes (interleukin-4 and -10) were further validated by fluorogenic quantitative RT-PCR.Results There were 432 (2.86%) differently expressed genes in tetramethylpyrazine group.Of which,307 genes were up-regulated and 125 genes were down-regulated.The function of some genes were well known.Conclusions Tetramethylpyrazine,which can depress inflammatory reaction,anti-adhesive molecule and improve immune systems,is a potential therapy for ulcerative colitis although more detailed curing targes of such medicine need to be further study. Key words: Colitis,ulcerative; Oligonucleotide array sequence analysis; Mice
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