Impact of Tart Cherry Anthocyanins and Dietary Carbohydrate Sources on Intestinal Tumorigenesis in APCMin/+ mice

Abstract

Consumption of tart cherry anthocyanins (TCA) has been demonstrated to reduce the development of intestinal tumors in APCMin/+ mice, whereas high‐sucrose diets have been shown to increase intestinal tumor development in this model. The aim of this study was to determine the efficacy of TCA to reduce intestinal tumorigenesis in APCMin/+ mice consuming diets rich in sucrose and other dietary carbohydrates. Mice were assigned to six different modified AIN‐93G diets, which contained one carbohydrate source (starch, sucrose, or glucose) and TCA (0 or 800 mg/kg). To enhance tumor development, mice were injected for three consecutive weeks with azoxymethane (8 mg/kg) prior to treatment. Mice consuming the sucrose diet without TCA had the greatest adenoma burden in the colon compared to mice consuming sucrose‐TCA, glucose‐TCA, glucose, and starch (P<0.05). No statistically significant differences between other diets were observed in the colon. Mean tumor burden in mice consuming sucrose without TCA was 1.9 to 5.2 times higher when compared to all other diets. These results demonstrate that feeding TCA reduces the additional intestinal adenoma development observed in APCMin/+ mice consuming sucrose.Grant Funding Source: MSU Internal Funding