Impact of sulfate and iron oxide on bacterial community dynamics in paddy soil under alternate watering conditions

Abstract

Sulfate and iron oxides are often used as amendments in paddy soil, but their ecological risks for soil microbiomes are not well understood. Paddy soil amended with gypsum or hematite was incubated in laboratory microcosms under submerged (56 d) and subsequent dry (35 d) conditions. The soil bacterial community composition stabilized after 15–21 d of submergence and remained largely unchanged after redrying. The presence of OTUs related to facultative anaerobic bacteria (mainly Acidobacteria groups 7 and 16, Gemmatimonas, and unclassified bacteria) probably accounted for the limited variation in community composition after redrying, as suggested by random forest regressions. Redrying caused remarkable changes in the relative abundance of many bacteria putatively involved in soil reduction and oxidation. Gypsum and hematite did not stimulate sulfate or iron reduction after soil submergence. Although gypsum and hematite largely preserved the bacterial community composition, they significantly reduced the abundance and diversity of the total bacteria (by 3–12%), as well as the relative abundance of many putative bacterial reducers and oxidizers (by 17–100%), compared to the control. The results suggested the potential hazardous effects of sulfate and iron oxide on the bacteria in paddy soil, which should be considered before applying these amendments.