Abstract
Two up-flow blanket filter (UBF) reactors were employed to treat synthetic wastewater with different substrate concentrations and nitrogen load rates (NLR) for 178days. During days 0–60, higher influent NLR of R2 (0.21–0.58kg·m3·d) slowed down the formation of anammox sludge compared with the lower NLR of R1 (0.18–0.31kg·m3·d). Difference in sludge color and nitrogen conversion rate indicated greater anammox activity of R2 than R1. During days 61–178, R1 and R2 achieved the maximum nitrogen removal rates (NRR) of 1.213 and 1.684kg/(m3·d) under the NLRs of 1.924 and 2.502kg/(m3·d), respectively. Furthermore, high-throughput sequencing showed that R2 (43.5%) had a higher proportion of anammox bacteria than R1 (37.8%) and less species. These results showed that after going through a higher NLR acclimation process during start-up period, stronger resistant capability against high impact nitrogen load and greater anammox activity were obtained by R2.
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