Impact of standardized physical exertion on oxidative stress biomarkers in exhaled breath condensate of patients suffering from severe chronic obstructive pulmonary disease - The PHAETON project

Abstract

Chronic obstructive pulmonary disease (COPD) is linked to increased oxidative and nitrative stress (RNOS) in the airways and systemic circulation. RNOS causes damage of fosfolipids and proteins in cellular membrane including airway epithelium. Exhaled breath analysis (EBC) can reflex intensity of RNOS in COPD. We compared concentration of RNOS biomarkers and anti-inflammatory lipoxin A4 (LXA4) in EBC in COPD patients and healthy controls before and after incremental shuttle walk test (ISWT), actigraphy was used to follow average daily steps 1 week before ISWT. In 29 patents with severe COPD (age 62±1,7, FEV1 48,8 ± 15,6%) and 17 age matched healthy controls were during 1 day performed EBC collection, EBC samples were frozen and analyzed by mass spectrometry with liqid chromatography. After EBC sampling subjects underwent ISWT, and then again gave EBC. Results: We tested 8-isoprostane (8-ISO), cysteinyl leucotriens (LTB4, LTC4) and LXA4. In COPD was 8-ISO 48.2±3.5, LTB4 45.8±2.3, LTC4 56.4±14.9 pg/mL and LXA4 18.5±8.9 pg/mL, in control group were results of RNOS lower, and LXA4 higher (all differences p Conclusion: The higher RNOS in EBC the lower ISWT and daily acitivity. COPD patients with lower oxidative stress production had better tolerance of physical activity.