Abstract
Several studies evidenced that the use of MACS (Magnetic-Activated Cell Sorting - Annexin V columns) for semen samples with high levels of DNA damage could be useful to reduce it (1). However, other studies demonstrated that there was no beneficts of using MACS. A few years ago, we published a retrospective study who showed that using MACS could improve the ongoing pregnancy rates (2) in a oocyte donation program. Thus, the present prospective controlled study aimed to evaluate the clinical outcomes using MACS in patients with sperm DNA fragmentation in a shared oocyte donation cycles. Prospective controlled study Our study population included 60 ICSI cycles (egg-shared donation program). Semen samples were previously studied to determine DNA fragmentation level by TUNEL assay. The normal value considered for TUNEL assay was 15%. Each oocyte cohort were split in two or three recipients. Semen samples with altered DNA damage were considered. Two groups were considered, altered level of DNA damage treated with MACS (Treatment, N= 34 cycles) and other recipient with altered level of DNA damage without MACS (Control, N= 26 cycles). Fertilization rate, positive ßHCG, clinical pregnancy, implantation rate and blastulation rate, were compared between groups. Statistical analysis was performed by ANOVA and chi-square as appropriate. Results are resumed in Table 1 (a p< 0.05) The use of MACS in patients with altered levels of DNA damage seems to improve the clinical outcomes. To have a better sperm quality improves embryo development and ongoing pregnancy when oocyte competence is not compromised.Tabled 1Table 1Treatment Group (MACS)Control GroupN°3426Recipients age43.1±2.942.8±2.6Male age43.9±3.343.4±3.5Oocyte Donor age27.8±2.626.6±2.9MII oocytes/Recipient6.66.5Fertilization rate (%)82.685.7DNA fragmentation (%)22.8±4.824.8±3.8Positive BHCG/transfer (%)59 (20/34)50 (13/26)Clinical pregnancy/transfer (%)56 (19/34)a31 (8/26)Implantation rate (%)41 (25/61)a23 (10/44)Blastulation rate (%)62a38 Open table in a new tab
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