Impact of smoking on plasma antioxidant defenses and urinary 8-hydroxy-2-deoxyguanosine in ischemic heart diseases

Abstract

Background: Oxidative stress is associated with various morbid conditions such as atherosclerosis and coronary artery disease. Cigarette smoking is said to be associated with high oxidative stress levels which may predispose to ischemic heart diseases (IHDs). Urinary 8-hydroxy-2-deoxyguanosine (8-OHdG) has emerged as a biomarker for oxidative stress induced deoxyribonucleic acid (DNA) damage. Aim and Objective: The present study aims at evaluating the plasma markers for oxidative stress and urinary 8-OHdG in smokers suffering from IHD. Materials and Methods: For the present study about 327 male subjects, including 127 healthy subjects and 200 consecutively admitted IHD patients were enrolled and were subsequently divided into two subgroups as smokers and non-smokers consisting of 100 non-smokers and 100 smokers, based on the history of smoking. The healthy control group consisted of 64 smokers and 63 non-smokers. All the subjects were evaluated for plasma malondialdehyde levels, total antioxidant status (TAS), and urinary 8-OHdG. Results: There was increased oxidative stress evident by strong negative correlation between 8-OHdG and TAS was observed in the present study. Conclusion: The urinary 8-OHdG may serve as a sensitive indicator of DNA damage in smokers.