Impact of Silver Nanoparticle Contamination on the Genetic Diversity of Natural Bacterial Assemblages in Estuarine Sediments

Abstract

Recent advancements in the nanotechnology industry have seen a growing interest in integrating silver nanoparticles (Ag-NPs) into consumer and medical products. To date, there has been little research into the toxicological impact that Ag-NPs will have when they enter the marine realm. Of particular concern are the possible effects of Ag-NPs on natural bacterial assemblages, given the antimicrobial activity of silver. In this study, estuarine sediment samples were dosed in triplicate for 20 days from a stock solution of Ag-NPs, with a final cumulative treatment of either 0 microg L(-1) (control), 25 microg L(-1) or 1000 microg L(-1). The experimental tanks were left for a further 10 days to allow for any recovery. Inductively coupled plasma-optical emission spectrometery (ICP-OES) of water and sediment samples confirmed that the Ag-NPs concentration in the aqueous phase decreased after each dosing and were transported to accumulate in the surface layer of the sediment (approximately top 3 mm). The overall concentration of Ag-NPs in the water column, however, increased steadily during the 20 days of dosing but decreased rapidly during the following 10 days without dosing. Nevertheless, the Ag-NPs did not have any impact on the prokaryotic abundance in the water column over the incubation period (ANOVA, P < 0.05). Environmental DNA was extracted from sediment samples and a two-step nested PCR-denaturing gradient gel electrophoresis (DGGE) approach, using PCR primers specific to the phylum Bacteria, was adopted to assess their diversity. Multivariate statistical analyses of presence/ absence matrices produced from DGGE profiles revealed negligible differences in bacterial diversity between treatments, suggesting that, under the selected experimental regime, Ag-NPs present little or no impact on estuarine sediment bacterial diversity. Possible reasons for this could include environmental factors, in particular the chloride ions in estuary water affecting the chemistry and behavior of Ag-NPs.