Impact of Response Surface Methodology–Optimized Synthesis Parameters on In vitro Anti-inflammatory Activity of Iron Nanoparticles Synthesized using Ocimum tenuiflorum Linn

Abstract

Iron nanoparticle (FeNP) synthesis was carried out using the aqueous leaf extract of Ocimum tenuiflorum Linn. (O. tenuiflorum) collected from the University Institute of Engineering & Technology campus, Maharshi Dayanand University, Rohtak. Response surface methodology (RSM) was used in the current study for optimization of three variables implying central composite design (CCD). The variables optimized for in vitro anti-inflammatory activity of synthesized FeNPs were temperature (25–45) °C, pH (2.0–8.0), and molarities (0.1–1).The anti-inflammatory activity of FeNPs ranged from 0 to 118.23 due to interaction among synthesis variables. The analysis of variance was significant at P < 0.05 and revealed that the established model was significant .The maximum in vitro anti-inflammatory activity of 118.25 was observed with 100 mg/mL concentration of FeNPs synthesized at 25 °C temperature, pH 8.0, and 0.1 M molarity. This study will form basis for use of FeNPs as a potential therapeutic solution for inflammatory problems.