Impact of remote postconditioning on rabbits with acute intestinal ischemia-reperfusion injury

Abstract

Objective To investigate the protective effect of remote postconditioning induced by a single 10-min episode femoral artery occlusion referfusion applied immediately before the oneset of superior mesenteric artery perfusion on acute intestinal ischemia-reperfusion injury (IRI).Methods Thirty-six healthy New Zealand white rabbits were randomly divided into three groups (n =12 in each group):control,IRI,and limb ischemic postconditioning (LIP).Tissue myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels were determined and the morphological changes in intestinal tissues were observed under the microscopy.Results MDA levels,MPO activity and the score of intestinal mucosal injury were significandy increased in IRI and LIP groups as compared with control group (P ＜ 0.01).MDA levels and MPO activity were significantly reduced in RLP group [(5.32 ± 1.54) μmol/L,and (1.94 ±0.51) U/100 g] as compared with IRI group [(8.93 ± 1.25) μmol/L,and (3.68 ± 0.45) U/100 g] (P ＜ 0.01).The score of intestinal mucosal injury in LIP group was lower than in IRI group (2.96 ± 0.64 vs.4.15 ±0.81).Conclusion Limb postconditioning protects the mice from acute IRI probable by decreasing the injury caused by oxygen free radicals and increasing the capability of antioxidation. Key words: Limb ischemia; Ischemic postconditioning; Ischemia-reperfusion injury; Acute intestinal ischemia