Abstract
Cumulus cells have a critical role in normal oocyte development and fertilization. Prunus cerasus is an anthocyanin rich berry and performs strong antioxidant activity. The present study set to determine if Prunus cerasus can affect expression of HAS2 (hyaluronan synthase 2) and progesterone receptor in Cumulus cells and its consequences outcome of the in vitro fertilization. 60 female and 15 male adult mice were used for mating and IVF (in vitro fertilization). Prunus cerasus extraction was added to the diet of female mice for 30 days. Ovulation induction and oocytes collection were done as routine. The cumulus cells were dissected apart, and the expression of progesterone receptor and HAS2 was detected using RT-PCR (real-time polymerase chain reaction). Fertilization rate was evaluated by IVF. All data were analyzed using t-test. Data was showed that expression of progesterone receptor and HAS2 in cumulus cells of mice that received prunus cerasus increased. Moreover, oocyte fertilization rate also increased significantly. Prunus cerasus as an antioxidant natural can become an important medication for improving oocyte quality and opening new opportunities for infertility treatment. It is concluded that Prunus cerasus consumption could improve fertility rate by increasing progesterone receptor and HAS2 activity in cumulus cells.
Highlights
Infertility is defined as inability to obtain fertility after one year of unprotected regular intercourse.[1,2,3,4] It has been reported that about 10% -15% of young couples suffer from infertility
Effect of Prunus cerasus on hyaluronic synthase2 (HAS2) and PGR expression in cumulus cells Expression of HAS2 and PGR mRNA in cumulus cells, isolated from mice oocyte, was assessed by quantitative real time PCR
The results revealed that the addition of Prunus cerasus to laboratory diet resulted in increase of expression of HAS2 and PGR gene in cumulus cells and fertility success rate
Summary
Infertility is defined as inability to obtain fertility after one year of unprotected regular intercourse.[1,2,3,4] It has been reported that about 10% -15% of young couples suffer from infertility. 40%-55% are due to female factors, 20%-30% are due to male factors, and 15%-17% are unexplained infertility.[1] Over the years, ART (assisted reproductive technology) has been a method for infertility treatment with different causes.[5,6] In order to increase the success rate of fertilization, several herbal and chemical supplemental factors have been used.[7] Because of less invasiveness and costliness nature of traditional medicine, recent research has focused on herbal use.[8,9] Anthocyanin as secondary metabolite group of flavonoids has attracted much attention.[10] Strong antioxidant activity was the best known property of flavonoids.[11] Antioxidant activity of anthocyanin is associated with a variety of health benefits including inflammation, cancer, atherosclerosis, and diabetes.[12] Recent studies have shown that reproductive processes may be influenced by hypothalamic-pituitarygonadal axis via scavenging free radicals.[13,14,15] interest in antioxidant has recently been intensified because of their possible effect on egg quality, fertilization, and pregnancy rates.[16,17] Antioxidant therapy has been attended to alleviate infertility, and dietary antioxidant has been beneficial for female reproductive disorder.[18] Fruits including Sour cherry (Prunus cerasus), containing anthocyanin and strong antioxidant activity, have attracted much attention.[19] Prunus cerasus anthocyanins have a vast range of biochemical and pharmacological effect, and have been recommended as nutritional supplement.[20] Granulosa cells consist of mural and cumulus cells in preovulatory follicles. Mural cells are in the follicle wall and cumulus cell in vicinal of oocyte.[21]
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