Abstract
BackgroundProtein supplementation improves physiological adaptations to endurance training, but the impact on adaptive changes in the skeletal muscle transcriptome remains elusive. The present analysis was executed to determine the impact of protein supplementation on changes in the skeletal muscle transcriptome following 5-weeks of endurance training.ResultsSkeletal muscle tissue samples from the vastus lateralis were taken before and after 5-weeks of endurance training to assess changes in the skeletal muscle transcriptome. One hundred and 63 genes were differentially expressed after 5-weeks of endurance training in both groups (q-value< 0.05). In addition, the number of genes differentially expressed was higher in the protein group (PRO) (892, q-value< 0.05) when compared with the control group (CON) (440, q-value< 0.05), with no time-by-treatment interaction effect (q-value> 0.05). Endurance training primarily affected expression levels of genes related to extracellular matrix and these changes tended to be greater in PRO than in CON.ConclusionsProtein supplementation subtly impacts endurance training-induced changes in the skeletal muscle transcriptome. In addition, our transcriptomic analysis revealed that the extracellular matrix may be an important factor for skeletal muscle adaptation in response to endurance training. This trial was registered at clinicaltrials.gov as NCT03462381, March 12, 2018.Trial registrationThis trial was registered at clinicaltrials.gov as NCT03462381.
Highlights
Protein supplementation improves physiological adaptations to endurance training, but the impact on adaptive changes in the skeletal muscle transcriptome remains elusive
Protein feeding following endurance exercise has shown to affect Messenger ribonucleic acid (mRNA)-specific pathways involved in extracellular matrix, myogenesis, immunogenic response, and energy metabolism [15], suggesting that repeated post-exercise endurance protein feeding may enhance the adaptive
We have recently demonstrated that protein supplementation during endurance training enhances physiological adaptations, where the major part of the adaptations was observed during the first 5-weeks of the 10-weeks training intervention [16]
Summary
Protein supplementation improves physiological adaptations to endurance training, but the impact on adaptive changes in the skeletal muscle transcriptome remains elusive. The present analysis was executed to determine the impact of protein supplementation on changes in the skeletal muscle transcriptome following 5weeks of endurance training. Skeletal muscle is an extraordinary malleable tissue which is demonstrated by its rapid remodeling and adaptation to exercise training [1, 2]. Repetitive bouts of endurance exercise, e.g. endurance training, lead to various metabolic and morphological adaptations in skeletal muscle [3, 4]. Protein feeding following endurance exercise has shown to affect mRNA-specific pathways involved in extracellular matrix, myogenesis, immunogenic response, and energy metabolism [15], suggesting that repeated post-exercise endurance protein feeding may enhance the adaptive.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
