Abstract
Process parameters influencing l-glutaminase production by marine Vibrio costicola in solid state fermentation (SSF) using polystyrene as an inert support were optimised. Maximal enzyme yield (157 U/g drysubstrate) was obtained at 2% (w/w) l-glutamine, 35°C and pH 7·0 after 24h. Maltose and potassium dihydrogen phosphate at 1% (w/w) concentration enhanced enzyme yield by 23 and 18%, respectively, while nitrogen sources had an inhibitory effect. Leachate with high specific activity for glutaminase (4·2 U/mg protein) and low viscosity (0·966 Ns/m 2) was recovered from the polystyrene SSF system.
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