Impact of porcine follicular fluid during folliculogenesis as a supplement on the primary cell culture of oviductal epithelial cells

Abstract

The impact of varying concentrations of porcine follicular fluid (pFF) derived from three ovarian follicle types and produced during folliculogenesis on porcine oviductal epithelial cell (pOEC) culture was studied for 24 h using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Cells treated with pFF at a protein concentration of 500 µg/mL (from small- and large-sized ovarian follicles) and 600 µg/mL (from medium-sized ovarian follicles) showed the highest viability, which was significantly different from that observed in the control group (p<0.05) and not significantly higher than that observed in the positive control group.This study demonstrated that the impact of pFF on pOECs can be used as a model for biotechnological studies. Further, the study showed that instead of costly fetal calf serum, pFF produced during folliculogenesis can be used as a supplement in culture media to promote porcine oviductal epithelium cell growth and development.