Impact of polycation adsorption on efficiency and electroosmotically driven transport in capillary electrophoresis

Abstract

Adsorption of analytes to the walls of fused silica capillaries is common. This paper examines the quantitative impact of cationic polymer adsorption on electroosmotically driven transport, peak symmetry, and efficiency in capillary zone electrophoresis (CZE). Polycationic species were more strongly absorbed than polymers with a small number of positive charges. Recovery of proteins generally varied inversely with the isoelectric point pI). Adsorption of positively-charged species occurred preferentially at the capillary inet, generally within a few centimeters