Abstract
Platelet-rich plasma is a current subject of studies on chronic wound healing therapy due to possible pro-angiogenic effects. Microvascular compromise represents the major component in radiogenic wound healing complications. The effects of platelet-rich plasma on irradiated cells of the cutaneous wound healing process are poorly understood so far. In this study, the interaction of endothelial cells and adipose-derived stem cells in conjunction with treatment with platelet-rich plasma is investigated in the context of radiation effects. Therefore, the expression of surface-marker CD90 and CD31 was determined. Moreover, cell proliferation and viability after external radiation was analyzed with and without treatment by platelet-rich plasma.
Highlights
IntroductionChronic wounds after radiotherapy pose major challenges to head and neck surgery [1]
Chronic wounds after radiotherapy pose major challenges to head and neck surgery [1].The malfunction of wound healing in irradiated tissues is associated with fibrosis and decreased vascularity [1,2]
CD31 was used as a surface marker for microvascular endothelial cells
Summary
Chronic wounds after radiotherapy pose major challenges to head and neck surgery [1]. The malfunction of wound healing in irradiated tissues is associated with fibrosis and decreased vascularity [1,2]. Wound healing processes are based on a complex interaction network of cells and extracellular messenger molecules [3]. Decline in microvascularization represents a major component in radiogenic wound healing complications. Our group detected a reduction in the cell number of irradiated human dermal microvascular endothelial cells (HDMEC), adipose-derived stem cells (ASC) and the respective co-culture correlating with increasing radiation intensity [2]. Mesenchymal stem cells are suggested to support wound healing due to their production of multiple growth factors and cytokines which are
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