Impact of Phosphorylcholine Expression on the Adherence and Invasion of Streptococcus pyogenes to Epithelial Cells.

Hiroyuki Iuchi,Sakiko Toge,Takeshi Tokushige,Hisahiro Matsuzaki,Masaru Yamashita,Junichiro Ohori

doi:10.3390/microorganisms10030527

Hiroyuki Iuchi, Sakiko Toge + Show 4 more

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https://doi.org/10.3390/microorganisms10030527

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Journal: Microorganisms	Publication Date: Feb 28, 2022
Citations: 5	License type: CC BY 4.0

Affiliation: Kagoshima University

Abstract

Phosphorylcholine (PC) is a structural component of various pathogens and is involved in bacterial adhesion via the platelet-activating factor receptor (PAF-R). In this study, we investigated how PC expression affects cell adhesion and invasion of Streptococcus pyogenes (S. pyogenes). Eight clinical strains of S. pyogenes were cultured, and PC expression was measured using fluorescence-activated cell sorting. Bacterial adherence and invasion were examined using Detroit 562 cells. An anti-PC-specific monoclonal antibody (TEPC-15) was used to inhibit bacterial PC, and a PAF-R antagonist (ABT-491) was used to inhibit cellular PAF-R. The emm gene was amplified by the polymerase chain reaction with the standard primers. The level of PC expressed on the S. pyogenes surfaces differed in each strain and differed even in the same emm genotype. Adherence assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in S. pyogenes. Similarly, intracellular invasion assay experiments showed a significant negative correlation between TEPC-15 and ABT-491 inhibitory effects and PC expression in S. pyogenes. This study suggests that S. pyogenes is involved in cell adhesion and invasion by PC.

Highlights

Recurrent tonsillitis is a common disease encountered by otolaryngologists [1]
We investigated the effect of bacterial surface PC expression on the adhesion and invasion in epithelial cells in S. pyogenes
PC Expression and emm Genotype In S. pyogenes, PC was expressed in all strains, but the intensity differed in each strain (Table 1)

Summary

Introduction

Recurrent tonsillitis is a common disease encountered by otolaryngologists [1]. Viruses are the most common causes of recurrent tonsillitis, followed by bacteria, including Streptococcus pyogenes (S. pyogenes), Haemophilus influenzae, and Streptococcus pneumoniae [2].S. pyogenes is a species of Gram-positive bacteria that causes respiratory tract infections with mild to modest disease (tonsillitis and pharyngitis) and invasive and potentially life-threatening diseases (cellulitis, necrotizing fasciitis, and toxic shock syndromes) [3,4].Mortality from lethal S. pyogenes remains high in developed and developing countries [5].In addition, there are reports of S. pyogenes outbreaks in communities and hospitals [6].Early diagnosis is required, and a recent study reported that marker genes (spyCEP, ssa, sic, sdaB, speG) can be used as a rapid diagnostic tool for S. pyogenes [7]. Recurrent tonsillitis is a common disease encountered by otolaryngologists [1]. Viruses are the most common causes of recurrent tonsillitis, followed by bacteria, including Streptococcus pyogenes (S. pyogenes), Haemophilus influenzae, and Streptococcus pneumoniae [2]. S. pyogenes is a species of Gram-positive bacteria that causes respiratory tract infections with mild to modest disease (tonsillitis and pharyngitis) and invasive and potentially life-threatening diseases (cellulitis, necrotizing fasciitis, and toxic shock syndromes) [3,4]. Mortality from lethal S. pyogenes remains high in developed and developing countries [5]. There are reports of S. pyogenes outbreaks in communities and hospitals [6]. A recent study reported that marker genes (spyCEP, ssa, sic, sdaB, speG) can be used as a rapid diagnostic tool for S. pyogenes [7]

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Conclusion