IMPACT OF PHARMACOLOGICAL AGENTS ON INSULIN SECRETION CELLS TRANSPLANTED IN DIABETIC RATS

Abstract

Cyclosporin A is an immunosuppressive agent that improves survival of transplant. This exciting immunosuppressive agent was first used clinically in renal transplantation (Ferguson and Fidlus 1982).The aim of this study was the isolation , purification and transplantation of hamster pancreatic islet as xenograft transplantation for the treatment of diabetic rats. It is also aimed to study the effect of cyclosporin A as an immunosuppressive agent on some biochemical parameters e.g blood glucose and blood insulin levels to induce maximum suppression of mice immune system and consequently realizing maximum survival of transplanted islets. A total of 30 streptozotocin induced rat were randomized to receive islets xenografts from golden syrian hamsters by three different approaches ,the first group of ten rats received islets only in the renal subcapsular space, the 2nd of ten rats received islets in the renal subcapsular space and was given cyclosporin A in a dose of 30 mg/kg/day for three days only.The 3rd group of ten rats were received islets in the renal subcapsular space and were given cyclosporin A orally every day at a dose of 30 mg/kg/day for three days which was gradually decreased to 10 mg/kg/day. Non of rats of group 1 became normoglycemic. All rats of group 2 became normoglycemic for 228± ( range 12-36 days) . Only rats of group 3 enjoyed normoglycemic as long as cyclosporin-A was administrated. Consequantly, prolonged survival of islets xenografts may be achieved with administration of cyclosporin-A (Springer,et al., 2015).