Impact of pH on Cu Accumulation Kinetics in Earthworm Cytosol

Abstract

We studied the interaction between toxic stress and accumulation in the earthworm Aporrectodea caliginosa, as induced by different soil copper pools and soil constituents (especially pH). Earthworms were exposed in quartz sand, spiked soils, and field soils with different Cu concentrations and varying soil composition. The copper content in the earthworms was determined in the following: the cytosolic fraction, a granular fraction and a fraction consisting of tissue fragments, cell membranes and intact cells. The highest amount of Cu was found in the cytosolic fraction. The other fractions varied only slightly in response to changes in any of the copper pools in soil. Cytosolic copper was the best predictor of Cu availability to earthworms collected from soils at constant pH, as statistically significant correlations were obtained with pore water pCu at constant pH in earthworms exposed in quartz sand. This correlation was lost for cytosolic Cu concentrations in earthworms exposed to spiked soils and field soils at differing pHs. Instead, cytosolic copper correlated well to Cu in either pore water or solid phase. Soil pH not only plays an important role in the availability of metals and therefore on their uptake fluxes, but internal competition of Cu2+ and H+ at physiologically active binding sites also explained these apparent contradictions and increased the predictability of body burdens significantly.