Impact of organic and nanoparticle-selenium fortified larval weaning diet on survival, growth, gastrointestinal, and (GPx) activity of European Sea Bass (Dicentrarchus labrax) Larvae

Abstract

AbstractThe study involved three groups of six broodstock with a 1:1 male-to-female ratio. They were injected with 13 µg/kg LHRHa to stimulate spawning. We fed the broodstock three diets: a control diet without selenium, a diet containing 0.3 mg of nano-selenium /kg (N-Se), and a diet containing 4 mg of organic selenium /kg (O-Se). The broodstock diet consisted of equal parts of dry feed (45% protein), sardines, and squid. After being laid, the eggs were obtained from the collection ponds at the General Authority for Fisheries Development's fish hatchery and incubated until hatching. The larvae were given a micro diet containing 51.7% crude protein and live food (Rotifer and Artemia). Rotifer density increased from 5/ml to 15/ml by day 22 dph, Artemia nauplii increased from 0.2 to 4 ind./ml, from 7 to 35 dph then decreased to 0 at 55 dph, and micro-diets increased from 100-200 µ at 16 dph to 200-400 µ at 23-40 dph to 300-500 µ at 55 dph, and 500-1000 µ until the experiment ended. Three different diets in three replicates were tested: A micro diet free of selenium as a control diet (C), A micro diet with 0.3 mg of nano selenium (N-Se) kg-1, and A micro diet supplemented with organic selenium 4 mg (O-Se). Each group was taken from the three Broodstock groups respectively. Sixteen days after hatching, the three experimental micro diets were introduced to the larvae and continued for 76 days post-hatch. Larvae (260,000 per tank) were placed in fiberglass tanks with 4 m3 of water at a temperature of 19±2°C for optimal conditions. The larvae fed the N-Se diet had significantly better survival rates (75.0 ± 1.2 %), final body weight (240.0 ± 5.8 mg), weight gain (239.00 ± 5.8 mg), average daily gain (3.98±0.1mg), total length (39.0±0.06 mm), specific growth rate (9.133±0.04 %/day), feed intake (471.67±0.01 mg), and better feed conversion ratio (1.97±0.01) than the other groups (P < 0.05). The group provided with N-Se also had the highest levels of glutathione peroxidase enzyme (GPx) content in the whole larval body, whereas the control diet without Se supplementation recorded the lowest value of (14.8 ± 0.1 U/mg protein). The number and length of folds in the stomach and esophagus of sea bass post-larvae fed on diets fortified with selenium in both forms, nanoparticles or organic, showed a significant (P < 0.05) enhancement on the larval gastrointestinal (GI) tract. Moreover, selenium has an anti-inflammatory action in sea bass post-larvae. This study concluded that using N-Se as an antioxidant supports the growth and survival of European seabass D. labrax, gastrointestinal development and antioxidant efficacy during critical weaning.