Impact of needle insertion depth on the removal of hard-tissue debris.

Abstract

To evaluate the effect of depth of insertion of an irrigation needle tip on the removal of hard-tissue debris using micro-computed tomographic (micro-CT) imaging. Twenty isthmus-containing mesial roots of mandibular molars were anatomically matched based on similar morphological dimensions using micro-CT evaluation and assigned to two groups (n=10), according to the depth of the irrigation needle tip during biomechanical preparation: 1 or 5mm short of the working length (WL). The preparation was performed with Reciproc R25 file (tip size 25, .08 taper) and 5.25% NaOCl as irrigant. The final rinse was 17% EDTA followed by bidistilled water. Then, specimens were scanned again, and the matched images of the canals, before and after preparation, were examined to quantify the amount of hard-tissue debris, expressed as the percentage volume of the initial root canal volume. Data were compared statistically using the Mann-Whitney U-test. None of the tested needle insertion depths yielded root canals completely free from hard-tissue debris. The insertion depth exerted a significant influence on debris removal, with a significant reduction in the percentage volume of hard-tissue debris when the needle was inserted 1mm short of the WL (P<0.05). The insertion depth of irrigation needles significantly influenced the removal of hard-tissue debris. A needle tip positioned 1mm short of the WL resulted in percentage levels of hard-tissue debris removal almost three times higher than when positioned 5mm from the WL.