Impact of N-butyryl-l-homoserine lactone–mediated quorum sensing on acidogenic fermentation under saline conditions: Insights into volatile fatty acids production and microbial community

Abstract

Anaerobic fermentation is often inhibited under high salinity conditions. This study discovered a strong, positive association between N-butyryl-l-homoserine lactone (C4-HSL)–mediated quorum sensing (QS) and the production of volatile fatty acids (VFAs) under saline conditions. N-acyl-homoserine lactones were identified during acidogenic fermentation for VFA production. Only C4-HSL was detected at all salt concentrations, and a maximum C4-HSL concentration of 0.49 μg/L was observed at a salt concentration of 15 g/L. C4-HSL secretion was closely related to salinity, and a strong correlation was observed between C4-HSL and VFAs (p < 0.01), especially butyrate. Further experiments with C4-HSL addition indicated that exogenous C4-HSL promoted substrate hydrolysis and increased butyrate production by 1.5 times at 15 g/L NaCl. Microbial community analysis indicated that unclassified_f__Enterobacteriaceae and Clostridium_sensu_stricto_1, associated with QS genes and butyrate production, were positively associated with C4-HSL. This study demonstrates the positive effect of C4-HSL–mediated QS on acidogenic fermentation.