Impact of maternal physical activity during gestation on porcine fetal, neonatal, and adolescent ovarian development

Abstract

To determine how exercise from mid to late (days 40–104) gestation impacts offspring body, uterine and ovarian weight, and ovarian cell proliferation at three different developmental stages, Yorkshire gilts were either exercised by walking (EX) or not exercised (CON). In parity 1, ovaries and uteri were collected from the heaviest (H) and lightest (L) neonates and adolescent (6 mo) offspring. In parity 2, mothers were assigned the same treatment groups, and ovaries and uteri were collected from H and L fetuses on day 94 of gestation. Body weight was greater (P < 0.02) for H than L fetuses and neonates but not affected by EX treatment at any developmental stage. Ovarian weight in L but not H neonates was greater (P < 0.02) in EX than CON. Labeling index (LI; percentage of proliferating cells) was greater (P < 0.01) in cortex than medulla regions of fetal and neonatal ovaries. In fetal ovaries, EX enhanced LI (P < 0.01), and LI was greater (P < 0.01) in H compared with L offspring. In adolescent ovaries, LI was greatest (P < 0.01) in healthy antral and least in atretic antral follicles, and LI was greater (P < 0.01) in granulosa than theca cells of healthy antral follicles. Thus, exercise increased LI in fetal but not neonatal or adolescent ovaries. Although maternal exercise during gestation influences fetal and neonatal ovarian development, impacts on fertility remain unknown.