Abstract
Impact of low salinity on morphology and function of hemocytes in ark clam species Anadara kagoshimensis was investigated using light microscopy and flow cytometry. In control group the water salinity was adjusted to 19.6‰, and experimental group was maintained at 14.8‰ and 8.8‰. Two cell types, amebocytes and erythrocytes, were identified in control group of ark clams. Erythrocytes constituted the main type of the cells, and amounted to 92.3±3.9 %. Hyposalinity changed that proportion: the number of amebocytes decreased 2.7 times and number of erythrocytes increased 7.6 times. Morphometric characteristics of hemocytes didn’t show statistically significant changes. As far as salinity decreased, the number of erythrocyte shades in hemolymph increased (3.5 times at salinity 8.8‰) and, in hemocytes, the reactive oxygen species (ROS) production grew (3.5. times at salinity 8.8‰).
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