Impact of Leaf Development Stages on Polyphenolics Profile and Antioxidant Activity in Clausena lansium (Lour.) Skeels.

Xiaoxiao Chang,Yusheng Lu,Jishui Qiu,Arshad Mehmood Abbasi,Xinbo Guo,Jianping Pan,Zhixiong Lin

doi:10.1155/2018/7093691

Abstract

Clausena lansium (Lour.) Skeels, commonly known as “wampee,” is an excellent food ingredient of medicinal value. Effects of leaf developmental stages on the composition of phenolics, flavonoids, and antioxidant activity were investigated. Phenolics composition was studied using HPLC-PAD, whereas antioxidant activity was estimated by oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. Increase in bound flavonoids, quercetin, and cellular antioxidant activity was observed in bound and free fractions at different stages of leaf development. Predominantly, quercetin and ferulic acid contents were high in free and bound fractions of old leaves. In addition, phenolic components depicted highly significant positive association (p < 0.05) with antioxidant activity. Overall, old leaves of wampee have utility value similar to leaf buds, so they could be a more sustainable and economical source of bioactive compounds for commercial application in nutraceutical and pharmaceutical industries.

Highlights

Clausena lansium (Lour.) Skeels commonly known as wampee is an evergreen tree, which is endemic to China
The bound phenolic content was significantly increased from 166.0 ± 3.2 to 331.7 ± 10.5 mg gallic acid equivalent (GAE)/100 g, almost 2-fold increase with leaf developing
The results suggested that old wampee leaves would have the same utility value with leaf buds, and old leaves would be sustainable and economical resources for food industrial application

Summary

Introduction

Clausena lansium (Lour.) Skeels commonly known as wampee is an evergreen tree, which is endemic to China. It is commonly cultivated in different parts of southern China including Fujian, Guangdong, Guangxi, and Hainan. The neuroprotective potential of wampee extract for enhancing memory and to treat Alzheimer’s and Parkinson’s diseases has been reported [9,10,11,12]. Phytochemical compounds and antioxidant activity in wampee leaf have rarely been reported so far. Present study was intended to investigate the change in phytochemical compounds and antioxidant activities in the wampee leaves during different developmental stages of leaf

Materials and Methods

Results and Discussion

Conclusion

Conflicts of Interest