Impact of Irrigation Strategies on Abscisic Acid and its Catabolites Profiles in Leaves and Berries of Baco noir Grapes

Abstract

To understand the relationship among soil and plant water status, plant physiology, and the hormonal profiles associated with it, abscisic acid (ABA) and its catabolites [phaseic acid (PA), dihydrophaseic acid (DPA), 7-hydroxy-ABA, 8′-hydroxy-ABA, neophaseic acid, and abscisic acid glucose ester (ABA-GE)] in leaves and berries from wine grape cultivar Baco noir (Folle blanche × Vitis riparia) were analyzed. The experiment was conducted during the growing seasons 2006 and 2007 in an irrigation trial set up in a commercial vineyard located in Niagara-on-the-Lake, ON, Canada. ABA and its metabolites were quantified using liquid chromatography with ion trap combined with electrospray ionization-mass spectrometry. The hormonal profile indicated a direct relationship between the amount of ABA and climatic factors. The ABA varied between 582 and 4,026 ng g−1 dry matter (DM), DPA between 417 and 562 ng g−1, and ABA-GE between 337 and 2,764 ng g−1 DM. At many sampling times PA in the leaves was undetectable, and its highest concentration (260 ng g−1 DM) was at beginning of July 2007. ABA followed different catabolic pathways depending on the plant water status. ABA was likely catabolized by conjugation to form ABA-GE in treatments at higher water deficit levels, whereas in treatments with high water status, the oxidation pathway leading to DPA or PA was likely preferred. The ABA and ABA-GE concentrations in the berries at harvest showed high correlation with soil and plant water status.